英文名称 | Recombinant Enterokinase (rEK) |
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中文名称 | 重组肠激酶 (rEK) |
储存条件 | rEK in 50mM PBNa, pH 8.0, 0.5M NaCl and 50% glycerol should be stored at -20℃. Guaranteed stable for 3 years when stored properly. |
英文名称 | Recombinant Enterokinase (rEK) |
---|---|
中文名称 | 重组肠激酶 (rEK) |
储存条件 | rEK in 50mM PBNa, pH 8.0, 0.5M NaCl and 50% glycerol should be stored at -20℃. Guaranteed stable for 3 years when stored properly. |
Recombinant Enterokinase (rEK) is the catalytic subunit of bovine enterokinase, which is expressed by the yeast Pichia pastoris and purified to yield a high enzyme activity preparation. rEK recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide bond after the lysine residue. The enzyme can be used to cleave any fusion protein that carries this sequence. The enzyme is tagged C-terminal with a 6xHis Tag, which is easy to separate after enzyme cleavage.